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Anti-Bad [Y208] (DyLight 488标记)abcam抗体 (ab139836)55折促销

发布时间:2015-02-27 09:47 点击次数:

Overview

  • Product nameAnti-Bad [Y208] antibody (DyLight® 488)参阅全部 Bad 一抗 ...
  • Description
    Rabbit monoclonal [Y208] to Bad (DyLight® 488)
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm
  • Specificityab139836 does not cross-react with other Bcl-2 members.
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the N terminal of Human Bad (UniProt ID: Q92934).

  • Positive control
    • HeLa cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab139836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: 1/50.

Target

  • FunctionPromotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • Tissue specificityExpressed in a wide variety of tissues.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Post-translational
    modifications
    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • Cellular localizationMitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    see all

Anti-Bad [Y208] antibody (DyLight® 488) images