Anti-Bax [6A7] abcam原装抗体 (ab5714)55折促销

Anti-Bax [6A7] antibody images

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  Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody [6A7] (ab5714)This image is courtesy of an Abreview submitted by Dr Alwin Scharstuhl

  Immunofluorescence analysis of Human foreskin fibroblast cells, staining Bax with ab5714.

  Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin prior to being blocked in 1% BSA + 2% normal goat serum for 30 mins at 20°C. Samples were incubated with 5 µg/ml primary antibody for 45 mins at 20°C; the diution buffer was 1% BSA, 0.1% saponin, 0.05% NaN₃ in PBS. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (ab150116), dilution 1/1000, was used as secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody [6A7] (ab5714)This image is courtesy of an anonymous Abreview

  Immunofluorescence analysis of Human PMN cells staining Bax with ab5714.

  The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 2% BSA for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG (H+L) conjugated to Alexa Fluor® 488 (green) (ab150113) used at a 1/500 dilution. Counterstained with DAPI (blue).

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  Western blot - Anti-Bax antibody [6A7] (ab5714)Image courtesy of an anonymous Abreview.
  Anti-Bax [6A7] antibody (ab5714) at 1/2000 dilution + whole tissue lysate prepared from human islet tissue at 20 µg
  
  Secondary
  Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution
  developed using the ECL technique
  
  Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds

  Image courtesy of an anonymous Abreview.

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  Flow Cytometry-Bax antibody [6A7](ab5714)
  Overlay histogram showing HeLa cells stained with ab5714 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5714, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
   

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