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Anti-Bax [6A7] abcam原装抗体 (ab5714)55折促销

发布时间:2015-02-27 09:45 点击次数:

Anti-Bax [6A7] antibody images

  • Immunofluorescence analysis of Human foreskin fibroblast cells, staining Bax with ab5714.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin prior to being blocked in 1% BSA + 2% normal goat serum for 30 mins at 20°C. Samples were incubated with 5 µg/ml primary antibody for 45 mins at 20°C; the diution buffer was 1% BSA, 0.1% saponin, 0.05% NaN3 in PBS. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (ab150116), dilution 1/1000, was used as secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human PMN cells staining Bax with ab5714.

    The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 2% BSA for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG (H+L) conjugated to Alexa Fluor® 488 (green) (ab150113) used at a 1/500 dilution. Counterstained with DAPI (blue).

    See Abreview

  • Anti-Bax [6A7] antibody (ab5714) at 1/2000 dilution + whole tissue lysate prepared from human islet tissue at 20 µg

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution
    developed using the ECL technique

    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab5714 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5714, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
     

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