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Bcl-xL (54H6) Rabbit mAb (Alexa Fluor 488 标记抗体)CST原装抗体55折促销

发布时间:2015-02-27 09:48 点击次数:

Application Dilution Species-Reactivity Sensitivity Isotype
F 1:50 HumanMouseRatMonkey Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot using the unconjugated antibody.

Applications Key: F=Flow Cytometry

Protocols

Specificity / Sensitivity

Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SEM (blue) and K-562 (green) cells using Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) compared to nonspecific Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) #4340 (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2764 reacts with human, mouse, rat and monkey Bcl-xL protein. CST expects that Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Bcl-xL in these species.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).