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Anti-Occludin abcam抗体 (ab31721)55折促销

发布时间:2015-02-27 09:50 点击次数:

Overview

Application Notes
IHC-FoFr IHC-FoFr: Use at an assay dependent dilution. PubMed: 20978075
WB WB: 1/250. Detects a band of approximately 63 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Occludin peptide (ab34440). Abcam recommends using 3% milk as the blocking agent.
In-Cell ELISA In-Cell ELISA: Use at an assay dependent concentration. PubMed: 22689949
ICC/IF ICC/IF: Use a concentration of 1 - 2 µg/ml.
IHC-P IHC-P: 1/100. PubMed: 19828778
  • Product nameAnti-Occludin antibody参阅全部 Occludin 一抗 ...
  • Description
    Rabbit polyclonal to Occludin
  • Tested applicationsIHC-FoFr, WB, In-Cell ELISA, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Occludin.

     

    Anti-Occludin antibody images

    • All lanes : Anti-Occludin antibody (ab31721) at 1/250 dilution

      Lane 1 : Kidney (Human) Tissue Lysate
      Lane 2 : Trachea (Human) Whole Cell Lysate - adult normal tissue (ab29599)

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 59 kDa
      Observed band size : 59 kDa
      Additional bands at : 63 kDa (possible post-translational modification).
    • ICC/IF image of ab31721 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31721, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
    • Anti-Occludin antibody (ab31721) at 1 µg/ml (blocked with 3% milk) + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 59 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

    References for Anti-Occludin antibody (ab31721)

    This product has been referenced in:
    • Denis MC  et al. Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. PLoS One 8:e53725 (2013). WB ; Human . Read more (PubMed: 23372666) »
    • Chen JC  et al. Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production. Fertil Steril 100:1132-43 (2013). Read more (PubMed: 23849844) »